Giovanna Ghirlanda

Biodesign C room 545
TEMPE
Professor
Faculty
TEMPE Campus
Mailcode
1604
Professor
Faculty
TEMPE Campus
Mailcode
1604

Biography

Giovanna Ghirlanda is a professor of chemistry at Arizona State University. Her research interests are in chemical biology, protein chemistry, and protein engineering. Professor Ghirlanda received a BSc/MSc degree in Medicinal Chemistry and a PhD in Organic Chemistry from the University of Padova, Italy, with a thesis on the redox catalytic properties of supramolecular metallocomplexes.

Prior to joining ASU, she pursued postdoctoral work at the University of Pennsylvania with Professor William F. DeGrado (now at the University of California San Francisco), specializing on de novo design of peptides and proteins. Her research is in the area of chemical biology and focuses on the design of functional proteins. A major thrust is in the area of sustainable fuel production, designing protein-based hybrid metalloenzymes that catalyze hydrogen production and carbon dioxide reduction in mild conditions. Other projects in the lab include the design of antiviral lectins and work on protein folding in WW domains, in collaboration with Professor Banu Ozkan (Physics, ASU). 

 

Education

  • Ph.D. Organic Chemistry, University of Padova, Italy 1996
  • BSc/MSc. Medicinal Chemistry, University of Padova, Italy 1991

 

 

Research Interests

My group uses de novo design and protein engineering to prepare artificial model proteins that can perform a desired function. The purpose of this effort is twofold: first, by reproducing the main properties and functions of natural proteins in a model system, one can test iteratively hypotheses on the specific contribution of each amino acid to the protein activity; second, once the key principles are elucidated, it will be possible to design novel proteins with activities beyond those of natural proteins. Methods utilized include computational modeling, solid phase synthesis, molecular biology techniques and enzymatic screening.

Current projects in the lab are divided in three main areas:

Artificial metalloproteins for solar to fuel energy conversion

The conversion of solar energy to usable fuel requires the assembly of a specialized chain of reactions, each typically facilitated by a metallic active site. To obtain the necessary spatial organization, we designed a family of peptides that can self assemble into large complexes functionalized with specialized active sites. We are using a variety of artificial amino acid and natural ligands to engineer [Fe4S4] clusters, diiron clusters, and manganese sites in scaffolding peptides. The ultimate goal is to catalyze biomimetic reactions such as water splitting, CO2reduction, and hydrogen production.

Design of membrane proteins

Membrane proteins function as gateways to the cell, and are crucial in a number of processes related to energy transduction and cell signaling. We are interested in learning how to control protein-protein interactions and cofactor binding in the membrane. To that end we have designed a stable, membrane soluble protein that utilizes a metal cofactor, heme, as active site.

Engineering novel glycan-binding proteins

We are using protein engineering and directed protein evolution to design reagents capable of targeting glycans with high affinity and specificity. The ability of attaching sugars to proteins, called glycosylation, is the most ubiquitous post-translational modification in eukaryotic cells. It is known to have a crucial role in many biological processes, including the immune response, inflammation, and the early stages of bacterial and viral infections. Therefore, the development of specific glycan recognition domains has applications both in basic research and in the diagnosis and therapy of diseases. As part of this effort, we have recently discovered the molecular mechanism of action of a potent anti-HIV protein, cyanovirin, paving the way for the design of improved antiviral proteins.

We are members of the EFRC Center for Bio-Inspired Solar Fuel Production (http://solarfuel.clas.asu.edu/ ) and of the Center for Membrane Proteins in Infectious Diseases (MPID, http://cemilinks.asu.edu/mpid/ )

Presentations

  • Tien Olson, Selva Edwardraja, Marco Flores, Giovanna Ghirlanda and James P. Allen. Enzymatic activity of a Mn-binding peptide. International Photosynthesis Conference, St Louis (Aug 2014).
  • Ghirlanda G. Protein-based catalysts for hydrogen production. ACS national meeting (Aug 2014).
  • Ghirlanda G. Directed Evolution of Protein-Based Hybrid Catalysts for Hydrogen Production. Frontiers in Biophysics and Chemical Biology, UCSF (Aug 2014).
  • Sommer, Roy, Ghirlanda. Design of artificial metalloproteins. Gordon Conference Solar Fuels, (Feb 2014).
  • Dayn Sommer and Giovanna Ghirlanda. Design of ferredoxin mimics. Gordon Research Seminar Bioinorganic (Feb 2014).
  • Ghirlanda G. Direceted evolution of artificial hydrogenases. Gordon Conference Peptides (Feb 2014).
  • Ghirlanda G. "Directed Evolution of Protein-Based Hybrid Catalysts for Hydrogen Production". Gordon Conference Ppetides (Feb 2014).
  • Ghrlanda g. Design of artificial hydrogenases. Western photosynthesis conference (Jan 2014).
  • Ghirlanda G. session chair. Western photosynthesis conference (Jan 2014).
  • Dayn Sommer, Roy A., Schmidt R., Astashkine A., Ghirlanda G. De novo Design of Metal Binding Peptides as Fuel Generating Catalysts. GRS Bioinorganic (Jan 2014).
  • Ghirlanda G. Design of artificial hydrogenases. Western Photosynthesis Conference (Jan 2014).
  • G. Ghirlanda. Design of functional proteins: towards mimics of hydrogenase. DOE meeting (Jul 2013).
  • A. Roy, C. MAdden, A. Astashkine, G. Ghirlanda. Design of functional proteins: towards mimics of hydrogenase. Royal Society Protein Engineering Meeting, Chester, UK (Apr 2013).
  • G. Ghirlanda. Computational analysis and experimental validation of the glycan-protein interaction in Cyanovirin. Royal Society Protein Engineering Meeting, Chester, UK (Apr 2013).
  • G. Ghirlanda. Design of functional proteins: towards mimics of hydrogenase. Johns Hopkins University (Apr 2013).
  • G. Ghirlanda. Design of functional proteins: towards mimics of hydrogenase. Seminar, Virginia Tech (Mar 2013).
  • M. Faiella, A. Roy, D. Sommer, and Giovanna Ghirlanda. De novo design of artificial hydrogenases. L'Oreal Women in Science event, Paris, France (Mar 2013).
  • G. Ghirlanda. Design of functional proteins: towards mimics of hydrogenase. Seminar, University of Rochester (Feb 2013).
  • Anindya Roy (presenter), C. Madden, M. Vaughn, A. Astashkine, and G. Ghirlanda. De novo Design and synthesis of a peptide based functional mimic of [FeFe] hydrogenase". Gordon Research Seminar (Feb 2013).
  • Sai Kumar, G. Ghirlanda, and Claudio MArgulis. MD simulations of cyanovirin. Society for glycobiology (Nov 2012).
  • Ghirlanda, G. Design of functional proteins: towards hydrogen production. PEM6- international peptide engineering meeting (Oct 2012).
  • Justin Flory1,2,3, Sandip Shinde2,3, Su Lin3, Hao Yan2,3, Giovanna Ghirlanda2,3, Petra Fromme2,3. Nucleic Acid Driven Peptide Assembly. PEM6 (Oct 2012).
  • Anindya Roy, Chris Madden, Giovanna Ghirlanda. Design and synthesis of a peptide based functional mimic of [FeFe] hydrogenase. PEM6- international peptide engineering meeting (Oct 2012).
  • Anindya Roy, I. Sarrau, A. Astashkine, and G.Ghirlanda. De novo Design and Synthesis of Artificial [4Fe-4S] Binding Peptide Motif. Protein Society meeting (Aug 2012).
  • Woodrum B., Bolia A., Wang X., Oxkan B. and Ghirlanda G. Dissecting the Determinants for Dimmanose Binding in Cyanovirin-�-N. Protein Society meeting (Aug 2012).
  • Ghirlanda, G. Design of Tailor-Made Glycan Recognition Protein Modules based on Cyanovirin. Physics, chemistry, and biology of membrane proteins (May 2012).
  • Matyushev, D; Woodbury N., Beckstein O.,; Fromme P.; Girlanda G. Physics, chemistry, and biology of membrane proteins. Workshop (May 2012).
  • G. Ghirlanda. De novo design of functional protein assemblies: towards hydrogen production. Heraeus Foundation: Harvesting Light (Apr 2012).
  • Ghirlanda G. Design of functional proteins. Colorado School of Mines (Feb 2012).
  • Ghirlanda, G. Design of functional proteins. EFRC review (Feb 2012).
  • Sizemore S.M., Cope S.M, Shinde S, Ghirlanda G and Vaiana S.M. Transient tertiary contact formation in the CGRP neuropeptide revealed by nanosecond laser spectroscopy. Biophysical Society (Feb 2012).
  • Binder J., Shinde S., DeMunari S., Ghirlanda G., and Levitus M. Monitoring Dimerization of GpA Using FRET. Biophysical Society (Feb 2012).
  • Anindya Roy, Chris Madden, Giovanna Ghirlanda. Design and synthesis of a peptide based functional mimic of [FeFe] hydrogenase. Gordon Graduate Seminars- Bioinorganic Chemistry (Feb 2012).
  • Ruben M., Woodrum BW., and Ghirlanda G. Directed evolution of gp120 binding proteins. Society for glycobiology (Nov 2011).
  • Sai Kumar, G. Ghirlanda, and Claudio MArgulis. MD simulations of cyanovirin. Society for glycobiology (Nov 2011).
  • G.Ghirlanda. Design of functional proteins. Marie Curie Conference, Padova, Italy (Sep 2011).
  • G.Ghirlanda. Rational design of antiviral lectins. UCSF (Sep 2011).
  • G.Ghirlanda. Design of model hydrogenases. DOE HUB meeting (Sep 2011).
  • Sandip Shinde, J. Cordova, B. Woodrum, and Giovanna Ghirlanda. Modulation of function in Heme binding membrane protein. American Peptide society annual meeting (Jun 2011).
  • Anindya Roy, I. Sarrau, K. Redding, and G.Ghirlanda. De novo Design and Synthesis of Artificial [4Fe-4S] Binding Peptide Motif. American Peptide society annual meeting (Jun 2011).
  • Mathieu Walther, Anindya Roy, and G.Ghirlanda. Design of multiprotein assemblies. American Peptide society annual meeting (Jun 2011).
  • G.Ghirlanda, A.K.Jones. Design of artificial hydrogenases. DOE EFRC meeting (May 2011).
  • G.Ghirlanda. Design of functional proteins: towards redox catalysis. Rice University (Feb 2011).
  • G.Ghirlanda. Design of functional proteins: antiviral lectins. UT Houston Medical Center (Feb 2011).
  • G.Ghirlanda. Design of functional proteins. University of Miami (Jan 2011).
  • G.Ghirlanda. Artificial proteins for bioenergetic processes. Pacifichem (Dec 2010).
  • G.Ghirlanda. Multivalent interactions are crucial for CV-N activity. � Protein Society Meeting, San Diego (Jul 2010).
  • G.Ghirlanda. Structure-based design of artificial lectins. � NIH Annual AIDS meeting (Jun 2010).
  • G.Ghirlanda. Design of Functional proteins. Seminar at University of Wisconsin-Madison (Apr 2010).
  • G.Ghirlanda. Structure-based design of artificial lectins. Gordon Conference Peptides (Feb 2010).
  • G.Ghirlanda. Structure-based design of artificial lectins. Gordon Conference Peptides (Feb 2010).
  • G.Ghirlanda. Artificial proteins for bioenergetic processes. AIRE workshop (Nov 2009).
  • G.Ghirlanda. Artificial proteins for bioenergetic processes. University of Missouri-Columbia (Oct 2009).
  • G.Ghirlanda. Multivalency effects in antiviral lectins. Johns Hopkins University (Oct 2009).
  • G.Ghirlanda. Design of functional proteins. Wayne State University (Apr 2009).
  • G.Ghirlanda. Design of functional proteins. University of Delaware (Apr 2009).
  • G.Ghirlanda. Design of functional proteins. University of Michigan (Apr 2009).
  • G. Ghirlanda. Modulation of redox potential in model hemoproteins. NSF Workshop on Physical Organic Chemistry (Sep 2008).
  • G. Ghirlanda. Design of functional proteins. University of Arizona (Sep 2008).
  • G. Ghirlanda. Novel Heme-binding proteins. RMACS Salt Lake City (Jun 2008).
  • G.Ghirlanda. Design of functional proteins. SUNY Buffalo (Feb 2008).
  • Ghirlanda, Giovanna. Sweet entanglement. Regional ACS meeting, Tucson, AZ (Oct 2006).
  • Katiliene, Zivile, Bogani, Federica, Giomarelli, Barbara, Mori, Toshi, Fromme, Petra, Fromme, Raimund, Ghirlanda, Giovanna. Design and Crystal Structure of a Monovalent Mutant of CV-N Provides Insight into the Role of Multivalency. Protein Society meeting (Aug 2006).
  • Bogani, F, Chang, Y, Joshi, L, Ghirlanda, Giovanna. A miniature MAF analog with native-like activity. Protein society meeting (Aug 2006).
  • Cordova, J, Noack, P, Ghirlanda, Giovanna. Design of a membrane protein with peroxidase activity. Protein society meeting (Aug 2006).
  • Ghirlanda, Giovanna. Protein Society Meeting (Aug 2006).
  • Ghirlanda, Giovanna. Biopolymers. Gordon conference (Jun 2006).
  • Bogani, F, Chang, Y, Joshi, L, Ghirlanda, Giovanna. A miniature MAF analog with native-like activity. Protein design and function conference (Jul 2005).
  • Cordova, J, Noack, P, Ghirlanda, Giovanna. Design of a membrane protein with peroxidase activity. Protein design and function conference (Jul 2005).
  • Ghirlanda, Giovanna. Metalloprotein and Protein Design (Jul 2005).
  • Ghirlanda, Giovanna. Proteins. Gordon conference (Jun 2005).
  • Ghirlanda, Giovanna. Design of Functional Proteins. (Oct 2004).
  • Ghirlanda, Giovanna. Protein Society meeting (Aug 2004).
  • Ghirlanda, Giovanna. Bioorganic Chemistry. Gordon conference (Jun 2004).
  • Ghirlanda, Giovanna. Design of functional proteins. (Apr 2004).
  • Ghirlanda, Giovanna. Young Scientists Symposium on career choices. European Symposium of the Protein Society (Apr 2003).
  • Ghirlanda, Giovanna. Design of functional membrane proteins.
  • Ghirlanda, Giovanna. NOVEL MEMBRANE PROTEINS FOR REDOX CATALYSIS. Protein Society Eropean Symposium